The Anti-Human PD-L1 is a type-1 transmembrane protein used in the regulation of humoral and cellular immune responses. When it interacts with the receptor PD01, it provides inhibitory and stimulatory signals in regulating T-cell activation, along with tolerance to autoimmune disease, malignant transformation, tissue allografts, and pregnancy. It is primarily expressed in antigen-presenting cells, placenta, activated B and T cells, and various tumors, including diffuse large B-cell lymphoma, melanoma, and long, color, or rectum carcinoma.
More About It
The anti-PDL1 is designed for research purposes only. It has a clone called SP142, and the immunogen is a synthetic peptide from the human PD-L1 and C-terminus protein. The isotype is the Rabbit igG, and the epitope is undetermined. The molecular weight of the Anti-PDL1 is 32 kDa, and it has been tested on humans.
Applications And Procedures
It is designed for Immunohistochemistry applications only. You should use Formalin-fixed tissues embedded in paraffin to prepare the specimen and should use xylene or an appropriate alternative for deparaffinization.
When using the concentrated formulation, you should dilute using a ratio of 1:100, depending on your protocols and methods. To retrieve the antigen, you will need to boil your tissue section in an EDTA buffer with a pH of 8.0 for approximately 10 minutes, cooling to room temperature for 20 minutes. Incubation periods are 10 minutes while at room temperature.
The positive control is the placenta, and the cellular localization is the membrane.
Formulations
You can find the Anti-PDL1 Antibody in multiple forms, including 7.0 ml, 1.0 ml, 0.5 ml, and 0.1 ml. All of which are placed in a PBS/1 percent BSA buffers with a pH of 7.6.
The Anti-PDL1 is designed for use in the membrane, primarily the placenta. Visit Spring Bioscience now for more information or to purchase this for your laboratory.
